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. 2005 Oct;167(4):1149–1159. doi: 10.1016/s0002-9440(10)61203-5

Figure 5.

Figure 5

Ets-1 induction of PDGF-Rα is abolished by mutation of the proximal Sp1 (−61G10−52) element in PDGF-Rα promoter. This site (−61G10−52) mediates Sp1-inducible PDGF-Rα expression.24 Cells were transfected with 10 μg of pLuc-a2 or pLuc-a2.mSp1 and 1 μg of pcDNA3-Ets-1 or pcDNA3. Firefly luciferase was determined in cell lysates after 24 hours. The y axis represents the ratio of Firefly luciferase activity over Renilla to normalize for transfection efficiency. The result is representative of at least two independent observations.