Figure 5.

3-NT is strongly expressed in mesopontine and forebrain cholinergic terminal projection sites in Tg2576 mice. There is minimal 3-NT immunoactivity in the hippocampus of 12-month-old WT mice (A and C). In contrast, there is intense 3-NT immunolabeling in the hippocampus of 12-month-old Tg2576 (B and D, which are shown at higher power than the images in A and C to illustrate the nature of the increased 3-NT immunolabeling). Similarly, there is minimal 3-NT immunolabeling in the lateral hypothalamus of 12-month-old WT mice (E), but intense 3-NT immunolabeling in the lateral hypothalamus of 12-month-old Tg2576 mice (F). Average number of 3-NT-positive neurons/matched slice/genotype in the hippocampus and hypothalamus of 12-month-old Tg2576 mice compared to age-matched WT littermate controls (G). Percentage of 3-NT-positive neurons in Tg2576 mice is compared to non-Tg mice (H). ***P < 0.0001.