Table 1.

Markers Tested and Immunohistochemical Detection Methods

Marker	Marker description	Dilution	Pretreatment	Buffer	Detection
CD1a*	MHC-like molecule, expressed on immature and mature myeloid DCs	1:5	Microwave	Citrate (pH 6)	Envision
Langerin*	C-type lectin, immature Langerhans cell marker	1:50	Autoclave	Citrate (pH 6)	Labvision
CD83*	Immunoglobulin superfamily member, DC maturation marker	1:25	Autoclave	Citrate (pH 6)	Envision
CD14*	Lipopolysaccharide co-receptor, expressed on myelomonocytic cells	1:25	Autoclave	Citrate (pH 6)	Envision
CD68†	Lysosome-associated molecule, macrophage marker	1:400	Microwave	Citrate (pH 6)	ABC method
S100†	Intracellular calcium-binding protein, expressed on activated Langerhans cells and myeloid DCs in lymph nodes	1:400	Protease§		ABC method
CD3†	T-cell receptor-associated signaling complex	1:100	Microwave	Tris/EDTA (pH 9)	Envision
MOPC21‡	Isotype control antibody	1:100	Autoclave	Citrate (pH 6)	Envision

^*Novocastra, Newcastle upon Tyne, UK. 

^†Dakopatts, Glostrup, Denmark. 

^‡Organon Teknika-Cappel, Boxtel, The Netherlands. 

^§Ventana, Tuscon, AZ.