Skip to main content
. 2005 Nov;167(5):1349–1359. doi: 10.1016/S0002-9440(10)61222-9

Figure 2.

Figure 2

Effect of ischemia over time on MMP-2 and MMP-9 levels both total and active for 30 days after onset of hindlimb ischemia. MMP-2 and -9 levels were assessed by quantitative bioactivity assays of muscle tissue taken from the ischemic and nonischemic limbs of wild-type mice on days 0 (pre-op), 1, 3, 7, 14, and 30. For both MMP-9 and MMP-2, total levels increased markedly within 3 days and then diminished by 30 days after surgery compared with nonischemic calf muscle. Active MMP-9 and MMP-2 remain markedly elevated in the ischemic calf muscle compared with the nonischemic muscle between 3 and 14 days after surgery when reperfusion by LDI was most marked. A: Total levels of MMP-2 including both the latent and the active forms in the ischemic and nonischemic muscle tissue (n = 8) at each time point. The error bars in the nonischemic data are narrow and obscured by the associated data point. B: Level of active MMP-2 present in the ischemic and nonischemic muscle tissue (n = 8) at each time point. The error bars for the nonischemic data do not exist because active MMP-2 was not detectable in the nonischemic calf muscle. C: Represents the total level of MMP-9, including both the latent form and the active forms, in the ischemic and nonischemic muscle tissue (n = 8) at each time point. D: Represents the level of active MMP-9 present in the ischemic and nonischemic muscle tissue (n = 8) at each time point. For A through D the asterisk denotes a significant difference (P < 0.05) between the ischemic and the contralateral nonischemic limb at the same time point. Error bars represent the SEM.