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VEGF receptor mRNA expression in NHEK cells. Expression of VEGFR-1 (A) and VEGFR-2 (B) mRNA was examined in NHEKs using RT-PCR. C: Expression of the housekeeping gene β-actin is also shown. As a positive control, HUVECs were subjected to the same procedures. The following are shown for each gene: 100-bp ladder (lane 1), NHEK cells (lane 2), HUVECs (lane 3), and a sample containing no reverse transcriptase (RT−) as a negative control (lane 4).
