Figure 3.

Microglial accumulation in APP/CCL2 mice. A: Ten-μm-thick slices of cortical (C) and hippocampal (H) regions of mice at 14 months of age (n = 6) were immunostained with anti-IBA-1 antibody and counterstained with hematoxylin. Arrows indicate IBA-1-positive cells in the brain regions. B: Quantification of IBA-1-positive cells in brain regions. *, P < 0.05 versus other groups as determined by analysis of variance and Newman-Keuls post hoc. C: TS-positive amyloid plaques (AP) were surrounded by IBA-1-positive microglial cells in both the hippocampal and cortical regions of APP/CCL2 or APP mice. D: Quantification of IBA-1-positive cells per μm² AP. *, P < 0.05 versus APP mice (n = 6) as determined by Student’s t-test. Original magnifications: ×200 (A); ×400 (C). Scale bars, 25 μm.