Table 1.
Summary of Antibodies Used for Immunohistochemistry and Immunoblotting
| Antibody | Epitope protein/amino acids | Type | Dilution | Company | Reference |
|---|---|---|---|---|---|
| Aβ | Aβ1-30 | P | 1:200 | Zymed | 52 |
| β-Actin | Slightly modified synthetic β-cytoplasmic actin N-terminal peptide | M | 1:100,000 | Sigma | 56 |
| ApoE | Purified mouse apoE from pooled mouse plasma high- and very low-density lipoproteins | P | 1:1000 | Biodesign | 55 |
| APP C-terminal | C-terminal 20 amino acids residue (751 to 770) of human APP | P | Calbiochem | 57 | |
| CD10 | Prokaryotic recombinant fusion protein corresponding to the external domain of the CD10 glycoprotein | M | 1:100 | Novocastra | 58 |
| CD11b/Mac-1 | T cell-enriched splenocytes from B10 mice | R | 1:50 | Serotec | 15 |
| CD11c | Purified from mouse ascites fluid | H | 1:100 | eBioscience | 59 |
| CD45 | Purified B cells from mouse lymph nodes | R | 1:500 | Serotec | 41 |
| 6E10 | Aβ3-8 | M | 1:500 | Signet | 56 |
| GFAP | GFAP isolated from cow spinal cord | P | 1:1000 | DAKO | 53 |
| IBA-1 | C-terminal portion of IBA-1 | P | 1:500 | * | 54 |
| IDE | Recombinant GST-fusion protein containing amino acids 97 to 273 of IDE | P | 1:1000 | Oncogene | 60 |
| MHC-II (I-A/I-E) | Activated C57BL/6 mouse spleen cells | M | 1:100 | BD PharMingen | 15 |
| PY-20 | phosphotyrosine-protein conjugate | M | 1:400 | Sigma | 56 |
| Synaptophysin | synaptosome preparation from rat retina | M | 1:1000 | Sigma | 57 |
*
Antibody from Dr. S. Kohsaka.
M, mouse monoclonal antibody; P, rabbit polyclonal antibody; R, rat monoclonal antibody; H, hamster polyclonal antibody.