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. 2005 May;166(5):1499–1514. doi: 10.1016/S0002-9440(10)62366-8

Figure 4.

Figure 4

Three-day-old hippocampal neurons expressing either GFP-P301L, GFP-V337M, or GFP-R406W and extracted with Triton X-100. The neurons were extracted using a Triton X-100-based protocol, fixed with cold methanol, and then double-immunostained with an anti-GFP antibody and a polyclonal anti-MAP2 antibody. In control cells transfected with GFP alone, the overexpressed protein was totally extracted (data not shown). In neurons expressing any of the three FTD-related mutant forms of tau, the GFP-tau remained attached to the microtubules during the extraction procedure. Microtubule-bound tau was found in the cell body, minor neurites, and the axon. On the other hand, the dendritic MAP, MAP2, was only attached to the microtubules located in minor neurites and the cell body. Scale bar, 5 μm.