Figure 4.

Expression of adherens and tight-junction proteins in melanoma, keratinocytes, and fibroblasts. Western blot analysis of protein extracts from melanoma cells (WM35, WM1366, and 1205Lu), keratinocytes (FK126), and fibroblasts (FF2441). Blots were probed with antibodies to α-catenin, β-catenin, plakoglobin (γ-catenin), p120catenin, occludin, claudin-7, and GAPDH. Experiments were performed at least three times and the figure shows representative blots. In each case, blots were stripped and reprobed for GAPDH to demonstrate equal protein loading.