Figure 9.

Knockdown of ZO-1 in melanoma cells fails to alter localization of E- or N-cadherin but reduces melanoma invasion and strength of cell-cell adhesion. A and B: Immunofluorescence images showing similar localization of E-cadherin in both control H1UG1 (not shown), N3 and Z11lentivirus-infected cells. C and D: The localization of N-cadherin is also similar in H1UG1 (not shown), N3 and Z11 lentivirus-infected WM35 cells (indicated by arrows). E: Gentle agitation of WM35 spheroids has no effect on N3-infected WM35 cells. F: However, Z11-infected WM35 spheroids are more easily disrupted. G: Preformed H1UG1 (not shown) and N3-infected WM35 control spheroids invade into the surrounding collagen after 96 hours. H: In contrast Z11-infected WM35 cell spheroids exhibit much reduced invasion into the collagen matrix. Scale bar, 100 μm.