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. 2006 Feb;168(2):685–694. doi: 10.2353/ajpath.2006.050295

Figure 5.

Figure 5

Regulation of Cat S mRNA and protein and elastase activity of the cell membrane fractions. Cell membranes prepared from HSMCs stimulated with or without IL-1β and IFN-γ were incubated with 1 mg/ml of elastin-Congo Red for 24 hours to determine the elastolytic activity in the presence or absence of LHVS (A) or analyzed by Western blot using antibody to Cat S (B). The data in B are combined with data on Cat S protein level. The fractions of cytosol and media from HSMCs stimulated with or without IFN-γ were analyzed for Cat S levels by Western blot (C). The expression of Cat S mRNA was detected by quantitative real-time RT-PCR in HSMCs cultured in presence or absence of IL-1β and IFN-γ. Data are derived from quantitative analysis of four independent experiments. *P < 0.05 and **P < 0.01 versus control; ###P < 0.0001 versus LHVS (−).