Figure 3.

Evaluation of Ca²⁺ and IP₃ levels. A: Measurement of intracellular IP₃ levels in cholangiocytes freshly isolated from rats of the different experimental groups, using the IP₃ [³H] assay (n = 6). Data are mean ± SEM of six experiments. *P < 0.05 versus the other groups. B: Assay of the intracellular Ca²⁺ levels in cholangiocytes freshly isolated from rats of the different experimental groups measured using fluo-3 AM and a Fluoroskan Ascent FL microplate reader (n = 6). Data are mean ± SEM of six experiments. *P < 0.05 versus NR, BDL, BDL + vagotomy + UDCA ± Gö6976, and BDL + vagotomy + TUDCA Gö6976.