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. 2006 Feb;168(2):398–409. doi: 10.2353/ajpath.2006.050126

Figure 5.

Figure 5

Evaluation of ERK1/2 and AKT phosphorylation. A: Measurement of ERK1/2 phosphorylation by immunoblot of whole cell lysates of cholangiocytes freshly isolated from rats of the different experimental groups. Top: Quantitative data of the changes in ERK1/2 phosphorylation (to compare NR cholangiocyte values, obtained in different experimental sets, data are expressed as percentage of BDL value, n = 3). Bottom: Representative images of the immunoblots for phosphorylated and constitutive ERK1/2 and β-actin. Data are mean ± SEM of three experiments. #P < 0.05 versus pERK1 of BDL, BDL + vagotomy + UDCA, and BDL + vagotomy + TUDCA; §P < 0.05 versus pERK2 of BDL, BDL + vagotomy + UDCA, and BDL + vagotomy + TUDCA. B: Measurement of AKT phosphorylation by immunoblot of whole cell lysates of cholangiocytes freshly isolated from the different experimental groups. Top: Quantitative data of the changes in AKT phosphorylation (to compare NR cholangiocyte values, obtained in different experimental sets, data are expressed as percentage of BDL value, n = 3). Bottom: Representative images of the immunoblot for phosphorylated and constitutive AKT and β-actin. Data are mean ± SEM of three experiments. *P < 0.05 versus pAKT of BDL, BDL + vagotomy + UDCA, and BDL + vagotomy + TUDCA.