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. 2006 Feb;168(2):551–561. doi: 10.2353/ajpath.2006.050480

Figure 2.

Figure 2

PTHrP and RankL expression in mammary tumors and r3T and 1029D6 cell lines. A: PTHrP expression was assessed by real-time PCR as described in Materials and Methods and was normalized to β2-microglobulin mRNA (β2M). The ratio of PTHrP/β2M was calculated for each sample. 4036T, 4009T, 1026T, and 1029T RNAs were prepared from DMBA-induced primary mammary tumors; 1029D6 and r3T represent total RNA prepared from the indicated cell lines grown in culture. Values shown are the mean and SD of triplicate determinations. B: RankL expression was determined and normalized as described above.