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Western blot of effects of TNF-α or IL-1β on MCP-1 output by E2 and E2 plus MPA-treated decidual cell monolayers. Confluent, passaged, leukocyte-free decidual cells were incubated for 7 days in 10−8 mol/L E2 or 10−8 mol/L E2 plus 10−7 mol/L MPA. The medium was exchanged for DM with the corresponding E2 or E2 plus MPA alone or with 1 ng/nl of TNF-α or IL-1β. After 24 hours of incubation, concentrated conditioned DM was subjected to Western blotting.
