Figure 5.

Expression of the milk β-casein protein in mammary glands of MMTV/Notch1^intra Tg mice. A: Mammary glands from pregnant (18 days) female mice were analyzed by IHC for expression of the milk β-casein protein, using rabbit anti-β-casein antibodies on tissue sections. B: Data were quantitated by image analysis (P < 0.0001). C: β-Casein/luciferase reporter assay in 293T cells in the presence (+) or absence (−) of Notch1^intra, Notch3^intra, or RBP-Jκ dominant-negative mutant (1.0 and 2.5 μg). The dotted line indicates that these experiments were done separately. Original magnifications, ×20.