Table 2.

Summary of CD163 Immunohistochemistry on HIVE and SIVE Brains

Clone	Tissue	Dilution	Pretreatment*	ABC or Polymer†	Staining
EDHu-1	Frozen SIVE	1:500	None	ABC or polymer	Perivascular
	Paraffin SIVE	1:250	None	ABC	Perivascular
	Paraffin SIVE	1:250	Microwave	ABC	Perivascular; scattered parenchymal
	Paraffin SIVE	1:250	None	Polymer	Perivascular; scattered parenchymal
	Paraffin HIVE	1:250	None	ABC	Perivascular; scattered parenchymal
	Paraffin HIVE	1:250	None	Polymer	Perivascular, scattered parenchymal
10D6	Paraffin SIVE	1:100	None	ABC	Perivascular (very weak)
	Paraffin SIVE	1:100	Boiling	ABC	Perivascular
	Paraffin SIVE	1:100	Microwave	ABC	Perivascular; scattered parenchymal
	Paraffin SIVE	1:100	Boiling	Polymer	Perivascular; scattered parenchymal
	Paraffin HIVE	1:100	Boiling	ABC	Perivascular; scattered parenchymal
	Paraffin HIVE	1:100	Boiling	Polymer	Perivascular; scattered parenchymal
GHI/61	Frozen SIVE	1:500	None	ABC	Perivascular
5C6-FAT	Frozen SIVE	1:1000	None	ABC or polymer	Perivascular

^*Pretreatment was performed either by microwave antigen retrieval in 0.01 mol/L sodium citrate buffer, pH 6.0, for 20 minutes or by heating in 0.01 mol/L sodium citrate buffer, pH 6.0, for 30 minutes at 95°C. 

^†ABC, avidin/biotin complex; polymer, peroxidase-labeled polymer conjugated with secondary antibodies.