Figure 11.

Effect of SDF-1/CXCR4 interaction on proliferation of cultured ICC cells and the influence of TNF-α were examined by using the proliferative reagent WST-8 and measuring the absorbance at 450 nm. Addition of 100 ng/ml of SDF-1 has no effect on proliferative activities of HuCTT-1 and CCKS-1 cells cultured for 48 hours. Additional treatment with 100 or 1000 U/ml TNF-α in this experiment had no influence on the proliferation of the cultured ICC cells for 48 hours either. Administration of AMD3100, an antagonist of CXCR4, had no effect on the proliferation of the cultured ICC cells. The data are presented as the mean ± SD. The assay for cell proliferation was performed three times in each experiment.