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. 2006 Apr;168(4):1155–1168. doi: 10.2353/ajpath.2006.050204

Figure 5.

Figure 5

A: CXCR4 and TNF-α mRNA were detected in carcinoma tissues obtained from central parts of ICC (three surgical specimens: ICC1, ICC2, and ICC3) by RT-PCR. B: TNF-α protein (17 kd) was detected in carcinoma tissues obtained from central part of all of three ICCs by immunoprecipitation using anti-TNF-α antibody and Western blotting. The right lane was a negative control using the second antibody only and the signals at 55 kd and 27 kd indicated goat immunoglobulins used for immunoprecipitation. C: By Western blotting, CXCR4 protein (46 kd) was detected in carcinoma tissues obtained from central part of all of three ICCs.