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. 2006 Apr;168(4):1155–1168. doi: 10.2353/ajpath.2006.050204

Figure 9.

Figure 9

Real-time quantitative PCR analysis for CXCR4 mRNA in two ICC cells (HuCCT-1 and CCKS-1) shows that on administration of TNFR1 or TNFR2 neutralization antibody, CXCR4 mRNA expression decreased 2.7-fold and 3.3-fold in HuCCT-1 and 2.3-fold and 1.6-fold in CCKS-1, respectively, compared to 1000 U/ml TNF-α alone (11.2-fold in HuCCT-1 and 8.2-fold in CCKS-1) but was still higher than the control (without TNF-α treatment). Simultaneous administration of anti-TNFR1 and anti-TNFR2 antibody (anti-TNFR1/2) suppressed CXCR4 mRNA expression 1.0-fold (HuCCT-1) and 0.9-fold (CCKS-1), respectively, relative to the control. TNF-α neutralization antibody suppressed more CXCR4 mRNA expression in HuCCT-1 and CCKS-1 cells treated with TNF-α to 1.2-fold and 0.9-fold, respectively, compared to the control. Control IgG did not suppress CXCR4 mRNA expression. *P < 0.05 versus 1000 U/ml TNF-α alone. **P < 0.05 versus 1000 U/ml TNF-α + anti-TNFR1 or -TNFR2 antibody.