Table 2.

Antibodies, Antigens, and Other Immunohistological Materials Used in This Study

	Source	Clone	Type of antibody	Animals immunized	Optimal dilution
Anti-TNF-α*	Santa Cruz Biotechnology		Poly	Goat (IgG)	1:200
Anti-SDF-1	Santa Cruz Biotechnology		Poly	Goat (IgG)	1:50
Anti-CXCR4	Santa Cruz Biotechnology		Poly	Goat (IgG)	1:50
Anti-CD68	DAKO	KP1	Mono	Mouse (IgG)	1:600
Anti-mast cell tryptase	DAKO	AA1	Mono	Mouse (IgG)	1:100
Anti-TNF-α†	R&D Systems		Poly	Goat (IgG)	2 μg/ml
Anti-SDF-1	R&D Systems	79014	Mono	Mouse (IgG)	50 μg/ml
Anti-TNFR1	R&D Systems	16805.21	Mono	Mouse (IgG)	10 μg/ml
Anti-TNFR2	R&D Systems	22221	Mono	Mouse (IgG)	10 μg/ml
TNF-α	PeproTech, London, UK				100, 1000 U/ml (=5, 50 ng/ml)
CXCR4	Santa Cruz Biotechnology				40 μg/ml
SDF-1	R&D systems				100 ng/ml
IL-1β	PeproTech				1000 U/ml (=100 ng/ml)
IL-4	PeproTech				1000 U/ml (=200 ng/ml)
IL-6	PeproTech				1000 U/ml (=100 ng/ml)
INF-γ	PeproTech				1000 U/ml (=50 ng/ml)
goat IgG	DAKO
Mouse IgG	DAKO
AMD3100	Sigma, St. Louis, MO				1 μg/ml

^*Used for immunohistochemical staining. 

^†Used for neutralization study.