Table 2.
Source | Clone | Type of antibody | Animals immunized | Optimal dilution | |
---|---|---|---|---|---|
Anti-TNF-α* | Santa Cruz Biotechnology | Poly | Goat (IgG) | 1:200 | |
Anti-SDF-1 | Santa Cruz Biotechnology | Poly | Goat (IgG) | 1:50 | |
Anti-CXCR4 | Santa Cruz Biotechnology | Poly | Goat (IgG) | 1:50 | |
Anti-CD68 | DAKO | KP1 | Mono | Mouse (IgG) | 1:600 |
Anti-mast cell tryptase | DAKO | AA1 | Mono | Mouse (IgG) | 1:100 |
Anti-TNF-α† | R&D Systems | Poly | Goat (IgG) | 2 μg/ml | |
Anti-SDF-1 | R&D Systems | 79014 | Mono | Mouse (IgG) | 50 μg/ml |
Anti-TNFR1 | R&D Systems | 16805.21 | Mono | Mouse (IgG) | 10 μg/ml |
Anti-TNFR2 | R&D Systems | 22221 | Mono | Mouse (IgG) | 10 μg/ml |
TNF-α | PeproTech, London, UK | 100, 1000 U/ml (=5, 50 ng/ml) | |||
CXCR4 | Santa Cruz Biotechnology | 40 μg/ml | |||
SDF-1 | R&D systems | 100 ng/ml | |||
IL-1β | PeproTech | 1000 U/ml (=100 ng/ml) | |||
IL-4 | PeproTech | 1000 U/ml (=200 ng/ml) | |||
IL-6 | PeproTech | 1000 U/ml (=100 ng/ml) | |||
INF-γ | PeproTech | 1000 U/ml (=50 ng/ml) | |||
goat IgG | DAKO | ||||
Mouse IgG | DAKO | ||||
AMD3100 | Sigma, St. Louis, MO | 1 μg/ml |
Used for immunohistochemical staining.
Used for neutralization study.