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. 2006 Apr;168(4):1335–1344. doi: 10.2353/ajpath.2006.051181

Figure 5.

Figure 5

Chronic ethanol abuse enhanced microglial reaction and caused oxidative stress. A: Microglial reaction (detected by Iba1 staining) was consistently higher in nonreconstituted ethanol (EtOH)-fed mice. PBL reconstitution and CTL brain infiltration further enhanced microglial responses in EtOH-fed mice. Semiquantitative analysis for Iba1 was performed by computer-assisted image analysis (Image-ProPlus; Media Cybernetics, Silver Spring, MD). B: Western blot analysis of nitrotyrosine levels in brain homogenates from control and EtOH-fed mice. Representative immunoblots of nitrotyrosine and internal standard α-actin are shown in blots and the ratios of nitrotyrosine staining to α-actin are shown in the histogram. Error bars represent SEM. *P < 0.008; **P < 0.02; #P < 0.05 (EtOH versus control).