Figure 6.

Simvastatin-induced apoptosis in A431 cells. A: Serum-starved A431 cells were treated with 0 to 60 μmol/L simvastatin for 24 hours, and cells were subjected to MTS assay as described in Materials and Methods. B: Serum-starved A431 cells were treated with 30 μmol/L simvastatin for 0 to 16 hours, and cells were subjected to MTS assay. Values represent the means ± SD of three independent experiments. C: Serum-starved A431 cells were treated either without or with 10 and 30 μmol/L simvastatin for 24 hours, and then cells were fixed and stained with propidium iodide, followed by sub-G1 analysis (values indicate the percentage of cells with sub-G1 DNA content). Values represent the means ± SD of three independent experiments. Similar results were obtained in three different experiments.