Figure 2.
ZO-1 protein in Ang II-treated podocytes shows normal levels of expression and maintains physical association with actin. a: Western blot analysis of ZO-1 in differentiated podocytes either unstimulated (control, lanes 1 to 3) or exposed to Ang II (10−7 mol/L for 1 hour) (lanes 4 to 6). b: Differential detergent experiments showing distribution of ZO-1 and actin in detergent-soluble (S) and -insoluble (P) fractions of control (lanes 1 and 2) and Ang II-stimulated podocytes (lanes 3 and 4). Numbers on the left indicate molecular values (× 10−3) of protein standards. In a and b, blots are representative of three and four experiments, respectively.