Figure 3.

A and B: Confocal micrographs of rat medial SMCs stained with phalloidin to label the actin cytoskeleton (red) and the focal adhesion protein paxillin (green). Several cells are evident in each micrograph. A: Control SMCs contained central actin stress fibers that terminated in paxillin-containing focal adhesions at the cell periphery (arrows). B: After 104 μmol/L doxycycline treatment, most actin filaments were found at the cell periphery, paralleling the cell membrane. Paxillin-rich focal adhesions were more concentrated around the peripheral edges of the cells (arrows). C and D: Confocal micrographs of rat medial SMCs stained with an antibody against phosphotyrosine. Control SMCs (C) exhibited phosphotyrosine staining at focal adhesions distributed evenly on the ventral surface of the cell (arrows) and also at the cell periphery (arrowheads). Doxycycline treatment (D) resulted in thicker focal adhesions that were more densely concentrated at the cell periphery (arrowheads). Magnification, ×60.