Figure 2.

Vinculin is a component of AJs in FLSs. Indirect immunofluorescence was conducted on FLSs that were cultured for 3 days on coverslips using phalloidin (green) to label F-actin, an antiserum to α-catenin (green), or an antibody to vinculin (red). A: The antibody to vinculin (red) labeled focal contacts (short arrows) as well as AJs (long arrow points to adhesion zipper with multiple clusters of AJs labeled as short parallel lines). B: The antiserum to α-catenin labeled AJs but not focal contacts. Co-localization of vinculin and α-catenin at AJs resulted in yellow labeling at sites of cell-to-cell contact in the merged picture (long arrow).