Figure 5.

Nf1^+/− but not WT mouse nerves contain EGFR⁺/p75⁺ cells. A and B: Schwann cells were dissociated from P0 to P1 mouse sciatic nerves and stained with anti-EGFR (green) and anti-p75 (red). A: Representative WT cell is p75⁺ but EGFR-negative. B: Nf1^+/− cells showing double staining for EGFR and p75. C: Graph shows quantification of percentage of EGFR⁺/p75⁺ cells in WT and Nf1^+/− nerves. The difference between Nf1^+/− and WT nerve was significant (P = 0.006; Nf1^+/−, n = 9; WT, n = 6). Scale bar, 10 μm.