Figure 5.

Invasive behavior of DOK cells is abolished by addition of monoclonal antibody against human collagen type IV in human homologous organotypic co-cultures. Human homologous organotypic co-cultures of neoplastic oral epithelium were constructed by growing for 10 days DOK cells on top of human fibroblast-containing matrices. The cultures were treated each second day starting from day 3 of co-culture with collagen IV antibody (100 ng/ml of culture medium). Parallel cultures treated with similar IgG subclass antibody served as negative controls. H&E staining is shown. Scale bar, 50 μm.