Table 1.
Epithelial Growth and Invasion Analysis of Reconstituted Human Oral Neoplastic Epithelium from Partially Transformed Oral Keratinocytes Grown on Top of Various Types of Connective Tissue Equivalents and in Different Culture Conditions
| Type of matrix | Growth parameters
|
Invasion parameters
|
||
|---|---|---|---|---|
| Epithelial thickness (μm) | Proliferation index (%) | Depth of invasion (μm) | Area of invasion (%) | |
| Human fibroblasts (hF) in collagen type I | 97.3 ± 6.7 | 21.2 ± 1.2 | 95.6 ± 7.1 | 45.8 ± 3.5 |
| Simple collagen type I | 61.1 ± 4.6 | 15.1 ± 0.4 | 14.1 ± 2.1 | 3.7 ± 0.8 |
| Sandwich model with simple collagen and hF in bottom layer | 88.7 ± 5.1 | 19.5 ± 1.1 | 38.7 ± 3.2 | 18.1 ± 2.1 |
| Simple collagen and conditioned medium from hF in monolayer | 73.3 ± 3.2 | 18.1 ± 0.9 | 11.4 ± 2.8 | 3.9 ± 0.7 |
| Simple collagen and conditioned medium from hF in organotypic co-cultures | 93.1 ± 7.3 | 20.9 ± 1.2 | 37.5 ± 4.1 | 14.5 ± 1.2 |
| Simple collagen and human growth factors in cocktail | 90.2 ± 6.1 | 27.0 ± 1.9 | 13.2 ± 3.7 | 4.1 ± 0.7 |
| Mouse fibroblasts (mF) in collagen type I | 84.6 ± 3.6 | 19.9 ± 1.4 | 11.5 ± 4.0 | 4.3 ± 1.0 |
| Sandwich model with mF and hF in bottom layer | 94.1 ± 3.1 | 20.3 ± 1.0 | 31.5 ± 2.6 | 21.7 ± 2.3 |
| mF in collagen and conditioned medium from hF in monolayer | 88.5 ± 4.1 | 21.5 ± 1.1 | 16.3 ± 2.8 | 7.4 ± 1.0 |
| mF in collagen and conditioned medium from hF in organotypic co-cultures | 90.8 ± 5.2 | 21.8 ± 1.0 | 27.2 ± 1.9 | 19.3 ± 1.2 |
| mF in collagen and human growth factors in cocktail | 110 ± 5.8 | 23.8 ± 1.3 | 17.2 ± 2.3 | 6.7 ± 0.9 |
| Rat fibroblasts in collagen type I | 81.1 ± 6.7 | 18.1 ± 1.8 | 15.6 ± 1.2 | 6.1 ± 0.5 |
Epithelial growth (total epithelial thickness and cell proliferation index) and invasion (depth and area of invasion) were assessed by histomorphometry and immunohistochemical staining. Data represent mean ± SEM of six different experiments.