Table 1.
Quantitation of FGFR mRNA Level in NHA and Human Glioma Cells
| mRNA copy number per 106 copies of β-actin mRNA
|
|||||||
|---|---|---|---|---|---|---|---|
| FGFR1c | FGFR1b | FGFR2c | FGFR2b | FGFR3c | FGFR3b | FGFR4 | |
| NHA | 11,815 ± 202 | 234 ± 10 | 4,347 ± 24 | 12 ± 6 | 125 ± 30 | 66 ± 8 | 250 ± 21 |
| U87 | 35,722 ± 916 | 1,864 ± 291 | 10,512 ± 1234 | 0 | 1 ± 1 | 2 ± 1 | 136 ± 2 |
| U105 | 11,399 ± 268 | 373 ± 6 | 3,211 ± 45 | 2 ± 2 | 12 ± 4 | 24 ± 9 | 164 ± 1 |
| U118 | 26,227 ± 1,682 | 2,886 ± 159 | 7,300 ± 518 | 3 ± 3 | 3 ± 2 | 9 ± 9 | 144 ± 8 |
| U251 | 35,971 ± 985 | 0 | 827 ± 87 | 27 ± 2 | 1,203 ± 164 | 4 ± 3 | 20 ± 2 |
| U373 | 1,848 ± 86 | 129 ± 16 | 2,105 ± 48 | 34 ± 4 | 262 ± 59 | 162 ± 21 | 184 |
Total RNA isolation and quantitative RT-PCR was performed as described in Materials and Methods. Individual standard ranges were used for different genes to ascertain that samples were within the range. All of the copy numbers were normalized to 106 copies of β-actin mRNA. Data are representative of three experiments.