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. 2006 Jun;168(6):1951–1959. doi: 10.2353/ajpath.2006.050781

Figure 3.

Figure 3

Transduction of HTR-8/SVneo cells by recombinant AAV-2 constructs (rAAV-2-LacZ and rAAV-2-GFP). A: Successful transduction of the Lac-Z transgene was measured by β-galactosidase activity along the y axis. HTR-8/Svneo cells were infected for 24 hours with 1–100 viral particles/cell of rAAV-2-LacZ in the presence or absence of 100 viral particles/cell of wtAd-5 (Ad), and significantly greater transduction rates compared to uninfected controls are indicated by asterisks. B: Successful infection and production of the GFP transgene was detected by green fluorescent staining. HTR-8/SVneo cells were infected for 24 hours with 100 viral particles/cell of rAAV-2-GFP in the absence of wtAd-5. Results are: from a light microscope (a and c), from a fluorescence microscopy (b and d), uninfected cells (a and b), and cells infected with rAAV-2-GFP (c and d).