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. 2006 Jun;168(6):1951–1959. doi: 10.2353/ajpath.2006.050781

Figure 4.

Figure 4

Adhesion and invasion of HTR-8/SVneo cells. A: Viability of HTR-8/SVneo cells was determined by measuring LDH leakage into cell culture medium 24 hours after infection with 15 to 1500 viral particles/cell of wtAAV-2 and/or 100 viral particles/cell of wtAd-5. B: Adhesion of HTR-8/SVneo cells to fibronectin-coated plates 24 hours after infection with 15 to 1500 viral particles/cell of wtAAV-2 and/or 100 viral particles/cell of wtAd-5 was compared to noninfected controls. Significant differences (P < 0.05) are indicated by an asterisk. C: Invasion assays were performed 24 hours after viral infection. Invasion was measured spectrophotometrically according to the optical density for crystal violet at 550 nm (y axis). Baseline invasion rates were determined for noninfected HTR-8/SVneo cells and 293 cells, and infection of HTR-8/SVneo cells by wtAAV-2 (150 viral particles/cell) and/or wtAd-5 (100 viral particles/cell) reduced invasion by ∼40%. Significantly different invasion rates between infected cells and controls (P < 0.05) were indicated by an asterisk.