Figure 13-6870.

Regulation of phase II and antioxidant genes by Nrf2. Nrf2^+/+ and Nrf2^−/− mice (2 months) were treated with corn oil or BHA (intragastric, treatment on days 1 and 3, 400 mg/kg body weight). Liver and spleen samples were taken out on day 4 and stored in RNALater (Qiagen). A: Total RNA from liver was prepared and analyzed by Northern blotting. Each group consists of three mice; each gene was analyzed with the same gel and exposure. Actin was used to ensure equal loading among samples. Arrows indicate genes with altered basal and/or inducible expressions in Nrf2-null mice. B and C: Total RNA from spleen was analyzed by real-time PCR. Data represent means ± SD from three samples. *P < 0.05.