Table 3.

Quantitation of PCNA Activity in 180-Day-Old VP following Neonatal DES

	% proliferation
	Epithelium	Stroma	Overall
WT control	24.91 ± 1.34	21.39 ± 5.52	25.15 ± 1.54
WT + DES	51.75 ± 6.03*†	19.49 ± 4.81†	44.55 ± 5.67*
ArKO control	33.22 ± 3.28*	34.77 ± 3.92*	33.53 ± 3.32*
ArKO + DES	48.38 ± 4.761*†	21.03 ± 3.00†	38.51 ± 3.91*

ArKO VP showed significant increases in levels of epithelial and stromal cell proliferation compared WT controls (P < 0.05), which was reflected in a significant overall increase in proliferation. Neonatal DES treatment elevated PCNA localization significantly in the epithelium of both WT and ArKO VP compared to respective controls, but had no effect on WT stroma and significantly reduced proliferation in ArKO stroma (P < 0.05), resulting in a significant overall increase in WT proliferation (P < 0.05). Data expressed as mean ± SEM. 

^*Significant comparison (P < 0.05) versus WT control. 

^†Significant comparison (P < 0.05) versus ArKO control.