Figure 1.
UCN and AM inhibit the production of inflammatory mediators by activated macrophages in vitro. A: Peritoneal macrophages were stimulated with LPS (1 μg/ml) in the absence or presence of different concentrations of AM or UCN. After different times (6 hours for TNF-α and 24 hours for IL-6, IL-12, IL-1β, RANTES, and MIP-2), pro-inflammatory mediators were assayed as described in Materials and Methods (n = 6). B: Peritoneal macrophages were stimulated with LPS (1 μg/ml) in the absence (control) or presence of AM or UCN (10−8 mol/L) for different times, and the inflammatory factors were determined in the supernatants (n = 6). *P < 0.001 versus untreated control mice.