Table 2.
Primers designed for the analysis of WNT4 expression by PCR
| Primers | Sequence (5'→3') | Function |
| R1 | AGCCTGACCATTGGAAGCCCTCT | 5'RACE T-PCR |
| R2 | GCCGCACAGAGTCCATCAC | 5'RACE |
| F1 | CATCGAGGAGTGCCAGTACCAGTTT | 3'RACE |
| F2 | GACGGTGGAACTGCTCGACTCTG | 3'RACE T-PCR |
| SMART IV | AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG | 5'RACE |
| CDS III | ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N (N = A, G, C, or T; N-1 = A, G, or C) | 3'RACE |
| 5' PCR Primer | AAGCAGTGGTATCAACGCAGAGT | 5'RACE |
| qF | GAAACCGACGGTGGAAC | qPCR |
| qR | AGGAGATGGCATAGACGAA | qPCR |
| 18S F | GATCCATTGGAGGGCAAGTCT | RT-PCR & qPCR |
| 18S R | CCAAGATCCAACTACGAGCTTTTT | RT-PCR & Qpcr |
| csF1 | GTCATCGGTGGGCAGCATCTC | Cross species cloning |
| csR1 | CGTGACACTTGCACTCCACCC | Cross species cloning |
F, forward primers in the 5' to 3'; R, reverse primers in the 3' to 5' direction. q, quantitative PCR primers for real time analysis; CDS, Smart IV and 5'PCR were synthesized by SIGAMA (Genosys) according to the manual of the SMART cDNA library construction kit from Clontech.