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. 2006 Oct 17;4(11):e340. doi: 10.1371/journal.pbio.0040340

Figure 1. Adaptive Tolerance Can Be Induced Independently of Regulatory Activity.

Figure 1

(A) Intracellular staining with antibodies to foxp3 (left panels) or an isotype-matched control (right panels) on naive 5C.C7 T cells (middle two panels) or antigen-adapted T cells (bottom two panels). Positive control staining in a polyclonal population of B10.A T cells marks approximately 19% of the CD4+ T cells (top two panels).

(B) A new cohort of naive 5C.C7 T cells expands similarly in B10.A, PCC+/+, CD3ɛ−/− hosts (open squares) even if they already harbor expanded 5C.C7 T cells (solid squares). Newly transferred 5C.C7 T cells were distinguished from the previous population using congenic markers (Ly5.1).

(C) Congenically marked Ly5.1+/+,5C.C7 T cells that expanded in a naive B10.A, PCC+/+, CD3ɛ−/− host (open squares) or a PCC+/+, CD3ɛ−/− host that had been injected with Ly5.2 +/+,5C.C7 T cells 28 d in advance (solid squares) were purified by FACS sorting (Ly5.1+, CD4+) and restimulated in vitro to measure IFNγ secretion.