Figure 7. Poor Differentiation and Survival Are Superimposed on Adaptive Tolerance in the Presence of Endogenous T Cells.

(A) The total recovery of Ly5.1⁺ 5C.C7 T cells from lymph nodes and spleen of recipient mice, various days after transfer into a T cell–replete (mPCC⁺,CD3ɛ⁺; open squares) host are compared with the recovery from a T cell–deficient (mPCC⁺,CD3ɛ^−/− ; filled squares) transfer recipient.

(B) Carboxy fluorescein succinimidyl ester profiles of transferred Ly5.1⁺ 5C.C7 T cells, 3 d after transfer into a lymphopenic (top) or T cell–replete (bottom) PCC transgenic host.

(C) Ly5.1⁺,5C.C7 T cells, recovered 9 d after transfer to a T cell–replete PCC transgenic (red line) or T cell–deficient PCC transgenic (blue line) host, were loaded with Indo-1 and stimulated as described in Materials and Methods. The influx of calcium, measured as the ratio of violet to blue fluorescence, is compared to that of naïve 5C.C7 T cells (green line).

(D) IL-2 or (E) IFN-γ secretion from sorted Ly5.1⁺,5C.C7 T cells restimulated in vitro with 10 μM MCC peptide and irradiated syngeneic splenocytes for 48 h, 15 d after residence in a T cell–replete (gray bars) or T cell–deficient (black bars) PCC transgenic hosts. Naïve 5C.C7 T cells (white bars) were used for comparison.

(F) Ex vivo IFNγ secretion by transferred T cells recovered 4 d after transfer to a T cell–replete (right) or T cell–deficient (left) PCC transgenic host. FACS plots are gated on CD4+, 7AAD-ve cells in the lymphocyte area.