Figure 1.

Real-time RT-PCR assay for cyclin D1a and b expression. Total RNA isolated from U266 MM cell line was serially diluted to obtain dilutions ranging from 10 to 0.625 ng. Cyclin D1a was amplified by RT-PCR using QRT D1a S and QRT D1a AS primers (400 nM) and cyclin D1b using QRT D1b S and QRT D1b AS primers (800 nM, Table 1). Amplifications are shown in logarithmic scale (left part). Threshold cycle (Ct) was plotted against quantity (ng) of RNA to obtain standard curve (right part). The slope of the curve is -3.32 indicating an amplification efficiency of 100%