Table 2.
Percentage of identity, as measured by the BLAST algorithm, at different taxonomic levels (inter/intra), and proportion of incorrect assignment of sequences at the specific and generic level (see text for details).
| DNA regions | taxonomic levels | identity (%) | wrong assignment (%) | |
|---|---|---|---|---|
| first occurrence | last occurrence | |||
| ITS1+5.8S+ITS2 | species | 95.75/97.20 | 6.59 | 45.73 |
| genus | 94.95/95.65 | 0.30 | 43.66 | |
| family | 94.35/95.24 | |||
| order | 92.98/93.12 | |||
| ITS1+5.8S | species | 97.24/97.43 | 0.84 | 43.65 |
| genus | 94.69/95.76 | 0 | 40.30 | |
| family | 93.02/94.27 | |||
| order | 92.07/92.54 | |||
| 5.8S+ITS2 | species | 95.48/97.21 | 3.57 | 40.77 |
| genus | 93.50/93.72 | 0.90 | 34.66 | |
| family | 91.38/93.21 | |||
| order | 90.75/91.79 | |||
| ITS1+ITS2 | species | 95.16/96.08 | 0.47 | 49.86 |
| genus | 93.98/94.42 | 1.13 | 46.28 | |
| family | 93.20/92.03 | |||
| order | 91.86/92.09 | |||
| ITS1 | species | 96.43/96.72 | 1.11 | 6.79 |
| genus | 93.39/95.77 | 0.02 | 39.64 | |
| family | 91.69/92.51 | |||
| order | 91.11/91.34 | |||
| ITS2 | species | 93.98/95.28 | 7.08 | 33.70 |
| genus | 93.78/93.10 | 0.10 | 51.68 | |
| family | 91.79/89.46 | |||
| order | 88.89/89.24 | |||
| 5.8S | species | 97.18/99.80 | 32.76 | 62.94 |
| genus | 96.30/98.45 | 36.04 | 64.62 | |
| family | 95.32/97.25 | |||
| order | 93.25/95.28 | |||
| rbcL | species | 97.62/99.73 | 3.69 | 16.95 |
| genus | 95.75/97.13 | 0.23 | 67.71 | |
| family | 91.43/95.92 | |||
| order | 88.81/92.65 | |||