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. 2005 Sep 12;360(1462):1889–1895. doi: 10.1098/rstb.2005.1720

Table 2.

Percentage of identity, as measured by the BLAST algorithm, at different taxonomic levels (inter/intra), and proportion of incorrect assignment of sequences at the specific and generic level (see text for details).

DNA regions taxonomic levels identity (%) wrong assignment (%)
first occurrence last occurrence
ITS1+5.8S+ITS2 species 95.75/97.20 6.59 45.73
genus 94.95/95.65 0.30 43.66
family 94.35/95.24
order 92.98/93.12
ITS1+5.8S species 97.24/97.43 0.84 43.65
genus 94.69/95.76 0 40.30
family 93.02/94.27
order 92.07/92.54
5.8S+ITS2 species 95.48/97.21 3.57 40.77
genus 93.50/93.72 0.90 34.66
family 91.38/93.21
order 90.75/91.79
ITS1+ITS2 species 95.16/96.08 0.47 49.86
genus 93.98/94.42 1.13 46.28
family 93.20/92.03
order 91.86/92.09
ITS1 species 96.43/96.72 1.11 6.79
genus 93.39/95.77 0.02 39.64
family 91.69/92.51
order 91.11/91.34
ITS2 species 93.98/95.28 7.08 33.70
genus 93.78/93.10 0.10 51.68
family 91.79/89.46
order 88.89/89.24
5.8S species 97.18/99.80 32.76 62.94
genus 96.30/98.45 36.04 64.62
family 95.32/97.25
order 93.25/95.28
rbcL species 97.62/99.73 3.69 16.95
genus 95.75/97.13 0.23 67.71
family 91.43/95.92
order 88.81/92.65