Table 3. H5–H2 and H5–H4 NOE magnitudes (%) of iduronic acid residues of octasaccharides A and B, in their free state and in the bound form, measured in buffer solution.
| Octasaccharide A | Octasaccharide B | ||||
|---|---|---|---|---|---|
| Mixing (ms) | Free ligand | Bound ligand | Free ligand | Bound ligand | |
| H5–H2 IdoA | 100 | 0 | 2.4 | ||
| 200 | 0 | 4.5 | |||
| 300 | 0 | 5.1 | |||
| H5–H4 IdoA | 100 | 2.6 | 4.0 | ||
| 200 | 6.1 | 9.2 | |||
| 300 | 7.2 | 10.8 | |||
| H5–H2 IdoA2S* | 100 | 0 | 3.5 | 0 | 3.0 |
| 200 | 0.9 | 7.0 | 0.4 | 7.8 | |
| 300 | 1.7 | 11.4 | 1.8 | 8.4 | |
| H5–H4 IdoA2S* | 100 | 1.9 | 3.4 | 2.9 | 4.1 |
| 200 | 4.8 | 7.1 | 5.5 | 8.3 | |
| 300 | 7.2 | 10.4 | 8.1 | 12.2 | |
| H5–H2 IdoA2S | 100 | 0 | 0 | ||
| 200 | 0.4 | 0 | |||
| 300 | 1.8 | 0.9 | |||
| H5–H4 IdoA2S | 100 | 1.9 | 2.5 | ||
| 200 | 3.2 | 6.2 | |||
| 300 | 5.5 | 9.5 | |||