Table 1. Normalized levels of relevant intracellular metabolites detected in vitro from S. nodorum strains SN15, mpd1-1, mdh1-71 and mpd1mdh1-102.

The metabolite levels were determined from each strain using three biological replicates. The normalized metabolite levels are arbitrary units that are calculated by dividing the area of the specific metabolite with that of the internal standard (ribitol), which is then divided by the fresh weight (g) and the amount of determined actin/g of fresh weight; the resulting number is then multiplied by 100 for simplification. The results are expressed as the means±S.D. nd, not detected.

		Normalized metabolite level
Metabolite	Strain…	SN15	mpd1-1	mdh1-71	mpd1mdh1-102
Mannitol		15.1±1.9	3.37±0.3	12.8±2.8	0.14±0.08
Glycerol		0.03±0.008	0.02±0.003	0.04±0.003	nd
Trehalose		0.4±0.1	9.3±0.6	0.6±0.1	5.92±1.64
Glucose 6-phosphate		0.005±0.001	0.2±0.02	nd	0.05±0.01
Fructose 6-phosphate		nd	0.02±0.001	nd	nd
Sorbitol		nd	0.1±0.02	nd	nd
Arabitol/xylitol		0.4±1.8	0.06±0.02	nd	nd