Table 2. Effect of statins, cerulenin and CVFM on BACE and γ-secretase activity.
Specific activity (SA), calculated from three independent experiments in which each experiment was performed in triplicate, is expressed as fluorescent units·h−1·(mg of protein)−1. ns, not significant; **P<0.01 and ***P<0.001.
| BACE activity (SA±S.D.) | γ-Secretase activity (SA±S.D.) | |
|---|---|---|
| Untreated | 34288±1824 | 759.8±39.6 |
| Lovastatin | ||
| 1 μM | 22707±1143*** | 729.6±8ns |
| 10 μM | 47685±1571*** | 925.5±26.8** |
| 50 μM | 27508±1394*** | 812.4±10.1ns |
| Simvastatin | ||
| 1 μM | 30186±171** | 1192.1±54.5*** |
| 10 μM | 29702±1437** | 629.2±2.58** |
| 50 μM | 40326±1632*** | 1231.9±35.1*** |
| Cerulenin | ||
| 5 μg/ml | 30329±1901ns | 1377.8±85.6*** |
| CVFM | ||
| 0.1 μM | 31692±678ns | 738.6±10.8ns |