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. 2006 Sep 27;399(Pt 2):297–304. doi: 10.1042/BJ20060873

Figure 4. Repression of P4.2 transcription by enforced expression of Brg1 in MEL cells.

Figure 4

(A) Western-blot analysis of transfected FLAG-tagged Brg1, total Brg1 (endogenous mouse+ transfected human), and endogenous Tal1, GATA-1 and Ini1 proteins in nuclear extracts from MEL cell transductants. (B) Quantitative real-time RT (reverse transcriptase)–PCR analysis of P4.2 and β-globin mRNA levels in undifferentiated Brg1- (B) and vector-transduced (V) MEL cells. (C) Northern-blot analysis of indicated mRNAs in Brg1-transduced MEL cells following incubation with 1.5% DMSO for 3 days. The levels of mRNAs in Brg1-transduced cells relative to vector controls are shown under autoradiographs. Ethidium bromide-stained 18 S and 28 S rRNA served as loading controls (bottom).