Table 1. Michaelis–Menten parameters for Spy1600 a series of 4-MU 2-N-acyl-2-deoxy-β-D-glucopyranosides.
The Taft electronic parameters (σ*) used for each N-acyl substituent were obtained from [35]. Abbreviations ND, no activity detected; [E]0, total enzyme concentration.
| Substrate | σ* | Km (mM) | Vmax·[E]0 (μmol·min−1·mg−1) | Vmax·[E]0/Km (μmol·mM−1·min−1·mg−1) |
|---|---|---|---|---|
| MU-GlcNAc | 0 | 3.58±0.34 | 28.78±2.70 | 8.04 |
| MU-GlcNAcF1 | 0.8 | 5.21±0.17 | 4.95±0.16 | 0.95 |
| MU-GlcNAcF2 | 2.0 | 14.07±0.70 | 0.60±0.03 | 0.04 |
| MU-GlcNAcF3 | 2.8 | ND | ND | ND |