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. 2006 Sep 27;399(Pt 2):305–314. doi: 10.1042/BJ20060757

Figure 4. Alignment of GAF-A domains from multiple trypanosomatid species with the cAMP-binding GAF-A domain of TbPDE2B, and expression of the NT+GAF-A domains of TcrPDEB1 and 2.

Figure 4

(A) Amino-acid-sequence alignment of GAF-A domains from T. brucei TbrPDEB2 (TbrB2), T. cruzi TcrPDEB1 (TcrB1) and TcrPDEB2 (TcrB2) and the GAF domains from the PDEs identified in other kinetoplastids: L. amazonensis (L. amaz), L. braziliensis (L. braz), L. mexicana (L. mex), C. fasciculata (C. fasc), T. evansi (T. evan) and T. rangeli (T. rang). The 11 putative cNMP-binding residues [40] are marked with arrows, residues that differ from the TbPDE2B/TbrPDEB2 cAMP-binding GAF-A domain are in boxes, and the NKFDE motif is shown with asterisks. (B) SDS/PAGE gel stained with Coomassie Blue and showing purified NT+GAF-A domains from TcrPDEB1 (lane 1), TcrPDEB2 (lane 2) and the catalytic domain of TcrPDEB1 (lane 3).