. 2006 Sep 27;399(Pt 2):305–314. doi: 10.1042/BJ20060757

Table 3. cAMP and MANT-cAMP hydrolysis and the K_i of dipyridamole (for cAMP hydrolysis) by the catalytic domain of TcrPDEB1, measured by ITC.

Hydrolysis of cAMP or MANT-cAMP by TcrPDEB1, or inhibition of TcrPDEB1 cAMP hydrolysis, using the PDE inhibitor dipyridamole were measured using ITC. The heats released/absorbed were plotted and analysed using the MicroCal® software to obtain K_m or K_i values. N/A, not applicable.

Substrate	Inhibitor	K_m (μM)	K_i (μM)	k_cat/K_m (M⁻¹·s⁻¹)
cAMP	−	2.8±0.6	−	7.9
MANT-cAMP	−	1.8±0.3	−	9.8
cAMP	Dipyridamole	−	10.8±0.2	N/A

Table 3. cAMP and MANT-cAMP hydrolysis and the Ki of dipyridamole (for cAMP hydrolysis) by the catalytic domain of TcrPDEB1, measured by ITC.

Table 3. cAMP and MANT-cAMP hydrolysis and the K_i of dipyridamole (for cAMP hydrolysis) by the catalytic domain of TcrPDEB1, measured by ITC.