Table 3. cAMP and MANT-cAMP hydrolysis and the Ki of dipyridamole (for cAMP hydrolysis) by the catalytic domain of TcrPDEB1, measured by ITC.
Substrate | Inhibitor | Km (μM) | Ki (μM) | kcat/Km (M−1·s−1) |
---|---|---|---|---|
cAMP | − | 2.8±0.6 | − | 7.9 |
MANT-cAMP | − | 1.8±0.3 | − | 9.8 |
cAMP | Dipyridamole | − | 10.8±0.2 | N/A |