Figure 6. Increase in intracellular glycation of TPI, PGI and 3-PGK from liver, kidney, brain and muscle of FN3K-deficient mice.

Tissue extracts were diluted in the lysis solution and BSA was added to standardize the protein concentration to 2 mg in 100 μl, which was then loaded onto the phenylboronate affinity columns. Non-glycated and glycated proteins were separated as described in the legend for Figure 5. The results are means±S.E.M. of four measurements for extracts from mice aged 1, 2, 4 and 6 months. The difference between the values obtained for the samples from the wild-type and Fn3k^−/− mice were compared using a Student's t test (***P<0.001; **0.001≤P<0.01; and, *0.01≤P<0.05).